Bioreactors For Animal Cell Culture

Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture.

Bioreactors for animal cell culture.

This article examines the underlying mechanisms involved in bubble associated cell damage and the methods available for controlling such damage. 4 1 1 and can be either mechanically driven stirred tank bioreactor or pneumatically driven bubble column air lift bioreactor. In general a conventional submerged type of bioreactor oxygenator with agitation system has been used in cell cultivations. Bioreactors play a key role in the field of biologics where they are used for the production of recombinant therapeutic proteins by large scale cultivation of animal cells.

The biological systems involved include enzymes microorganisms animal cells plant cells and tissues. There are several types of bioreactors including stirred tank airlift hollow fiber and rotary cell culture system rccs d. The same type of oxygenator as used for the homogeneous cultivation of microorganisms may be applied for plant cell cultivation and for micro carrier supported animal cell culture. Requirements for a bioreactor for animal cell culture 1 well controlled environment t ph do nutrients and wastes 2 supply of nutrients 3 gentle mixing avoid shear damage to cells 4 gentle aeration add oxygen slowly to the culture medium but avoid the formation of large bubbles which can damage cells on contact.

In one case a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. However despite this sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen especially on a large scale. There are three major types of homogeneous oxygenator systems used for. Home environment animal cell culture prev contents next bioreactors for suspension culture.

This process can either be aerobic or anaerobic these bioreactors are commonly cylindrical ranging in size from litres. Cell cultures are particularly susceptible to biological contamination. Microcarriers with adherent cells which were in principle an adaptation of homogeneous culture systems used for. Bioreactor is defined as a vessel that carries out a biological reaction and is used to culture aerobic cells for conducting cellular or enzymatic immobilization.

The gas sparging of culture broth causes damage to suspended animal cells. Animal cell culture bioreactors initially employed stirred tanks containing. Bioreactors for suspension culture belong to the group of dynamic systems compare sect. One bacterial spore or vegetative bacterial cell is all it takes to overgrow an animal cell culture.

The new bioreactor called the see saw bioreactor is ideal for the growth of cells with a sensitive membrane.